Post-Doc position in Molecular Biology and cell barcoding

Research collaboration with Curie Institute CBI - ESPCI Paris LBC Team - Pr Andrew Griffiths

ESPCI Paris - PSL

ESPCI Paris - PSL is a major institution of higher education (a French "Grande École d’ingénieurs"), an internationally renowned research center (6 Nobel Prizes), and a fertile ground of innovation for industry (3 start-ups created/year). ESPCI is a highly multidisciplinary environment with teaching and research in physics, chemistry and biology.

Prof. Andrew Griffiths’ Lab
The research activities of the Laboratory of Biochemistry team at ESPCI Paris-PSL, directed by Prof. Andrew GRIFFITHS, are based around droplet-based microfluidics, a powerful new ultrahigh-throughput system in which reaction volumes can be miniaturized by up to a million-fold compared to conventional assays in microtitre plates. This opens up exciting prospects for the development of extremely innovative systems with many applications in the Life Sciences. The successful candidate will join a highly multidisciplinary team, with experience spanning biology, chemistry and physics.

Project
The aim of the project is to develop a technology for selective single-cell transcriptomic (scRNA-seq) analysis of cells with a specific phenotype from a heterogeneous mixture. Microfluidic technics will be used to isolate and compartmentalize single cells in droplets. A molecular circuit called DNA tool box will be used for specific phenotyping of cells. The Post-Doc will work on developing live barcoding of cells of interest in droplets for downstream selective deep sequencing and validate it on infiltrating lymphocytes in solid tumour biopsies in collaboration with Sebastian Amigorena’s group (Institut Curie).

Requirements
We are seeking a highly motivated Post-Doc with strong experience in molecular biology (PCR, qPCR) and cell biology. Experience in microfluidics and bioinformatics are very appreciated.

Flexibility, autonomy, the ability to work in a highly multidisciplinary team and good interpersonal skills are essential.

Starting date : May 2022
Duration : 1 year
Salary : according to professional experience

Address your applications (CV + cover letter) by email to :
Prof. Andrew GRIFFITHS
ESPCI Paris – LBC Team
10 Rue Vauquelin - 75005 Paris